diff --git a/Marlin/src/feature/binary_stream.h b/Marlin/src/feature/binary_stream.h
index 80f26cc7ce..4bfa3998f1 100644
--- a/Marlin/src/feature/binary_stream.h
+++ b/Marlin/src/feature/binary_stream.h
@@ -39,7 +39,12 @@ inline int bs_read_serial(const serial_index_t index) {
 
 #if ENABLED(BINARY_STREAM_COMPRESSION)
   static heatshrink_decoder hsd;
-  static uint8_t decode_buffer[512] = {};
+  #ifdef BOTH(ARDUINO_ARCH_STM32F1, SDIO_SUPPORT)
+    // STM32 requires a word-aligned buffer for SD card transfers via DMA
+    static __attribute__((aligned(sizeof(size_t)))) uint8_t decode_buffer[512] = {};
+  #else
+    static uint8_t decode_buffer[512] = {};
+  #endif
 #endif
 
 class SDFileTransferProtocol  {