diff --git a/Marlin/temperature.cpp b/Marlin/temperature.cpp
index 8120e0d6c5..d7462bc99d 100644
--- a/Marlin/temperature.cpp
+++ b/Marlin/temperature.cpp
@@ -1483,8 +1483,15 @@ void Temperature::set_current_temp_raw() {
  */
 ISR(TIMER0_COMPB_vect) { Temperature::isr(); }
 
+volatile bool Temperature::in_temp_isr = false;
+
 void Temperature::isr() {
-  //Allow UART and stepper ISRs
+  // The stepper ISR can interrupt this ISR. When it does it re-enables this ISR
+  // at the end of its run, potentially causing re-entry. This flag prevents it.
+  if (in_temp_isr) return;
+  in_temp_isr = true;
+  
+  // Allow UART and stepper ISRs
   CBI(TIMSK0, OCIE0B); //Disable Temperature ISR
   sei();
 
@@ -1949,5 +1956,7 @@ void Temperature::isr() {
     }
   #endif
 
+  cli();
+  in_temp_isr = false;
   SBI(TIMSK0, OCIE0B); //re-enable Temperature ISR
 }
diff --git a/Marlin/temperature.h b/Marlin/temperature.h
index 182efd5645..e592148447 100644
--- a/Marlin/temperature.h
+++ b/Marlin/temperature.h
@@ -61,6 +61,8 @@ class Temperature {
                  current_temperature_bed_raw,
                  target_temperature_bed;
 
+    static volatile bool in_temp_isr;
+
     #if ENABLED(TEMP_SENSOR_1_AS_REDUNDANT)
       static float redundant_temperature;
     #endif