diff --git a/Marlin/src/lcd/dogm/u8g_dev_tft_320x240_upscale_from_128x64.cpp b/Marlin/src/lcd/dogm/u8g_dev_tft_320x240_upscale_from_128x64.cpp
index 82bef78244..205cafbccb 100644
--- a/Marlin/src/lcd/dogm/u8g_dev_tft_320x240_upscale_from_128x64.cpp
+++ b/Marlin/src/lcd/dogm/u8g_dev_tft_320x240_upscale_from_128x64.cpp
@@ -366,7 +366,7 @@ static const uint16_t ili9328_init[] = {
 static const uint16_t ili9341_init[] = {
   ESC_REG(0x0010), ESC_DELAY(10),
   ESC_REG(0x0001), ESC_DELAY(200),
-  ESC_REG(0x0036), 0x00E8,
+  ESC_REG(0x0036), TERN(GRAPHICAL_TFT_ROTATE_180, 0x00E8, 0x0028),
   ESC_REG(0x003A), 0x0055,
   ESC_REG(0x002A), 0x0000, 0x0000, 0x0001, 0x003F,
   ESC_REG(0x002B), 0x0000, 0x0000, 0x0000, 0x00EF,